ddddk tagged fusion protein overexpression e coli lysate Search Results


ddddk tagged fusion protein overexpression e coli lysate  (Bioss)
95
Bioss ddddk tagged fusion protein overexpression e coli lysate
Ddddk Tagged Fusion Protein Overexpression E Coli Lysate, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ddddk+tagged+fusion+protein+overexpression+e+coli+lysate/Tags+Positive+Control+Whole+Cell+Lysate/pmc09278077__mmc5-425-65-62
Average 95 stars, based on 1 article reviews
ddddk tagged fusion protein overexpression e coli lysate - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
human alpha synuclein overexpressioin plasmid  (Sino Biological)
91
Sino Biological human alpha synuclein overexpressioin plasmid
(A) The BZ555 strain was pretreated with 6-OHDA (30 mM) for 1 h then transferred to the plates with 5-Fluorouridine (0.04 mg/mL) and indicated concentrations of Rg3 or L-DOPA (10 µM) for 72 h. The fluorescence intensity of dopaminergic neurons was measured. (B) The eggs of NL5901 strain were treated with indicated concentrations of Rg3 or L-DOPA (10 µM). After growing to the L4 stage, the nematodes were treated with 5-Fluorouridine (0.04 mg/mL) for 12 days. The fluorescence intensity of <t>α-synuclein</t> protein in nematodes was measured. (C) The N2 strain was pretreated 6-OHDA (30 mM) for 1 h then transferred to the plates with Rg3 (90 µM) for 36h. The cardiolipin content was measured by NAO (10 μM) fluorescence signaling. (D-E) The BZ555 strain was cultured for three generations on culture plates with bacteria containing the indicated RNAi in the presence of Rg3 (90 µM). (D) Then the fluorescence intensity of dopaminergic neurons was measured. (E) The BZ555 strain was pretreated with 6-OHDA (30 mM) for 1 h then transferred to the plates with Rg3 (90 µM), 5-Fluorouridine (0.04 mg/mL) and cardiolipin (100 µg/ml) as indicated for 72 h. (F) The SJ4100 strain was treated with 6-OHDA (30 mM), 5-Fluorouridine (0.04 mg/mL) and Rg3 (90 µM) as indicated for different durations. The fluorescence representing UPR mt was measured. (G) The SJ4100 strain was treated with Rg3 (90 µM) and 6-OHDA (30 mM) as indicated for 12 h, the fluorescence representing UPR mt was measured. (H) The BZ555 strain fed with hsp-6 RNAi was pretreated with 6-OHDA (30 mM) for 1 h then transferred to the plates with 5-Fluorouridine (0.04 mg/mL) and Rg3 (90 µM) as indicated for 72 h. (I) The BZ555 strain fed with hsp-6 RNAi was pretreated with 6-OHDA (30 mM) for 1 h then transferred to the plates with 5-Fluorouridine (0.04 mg/mL), Rg3 (90 µM) and cardiolipin (100 µg/ml) as indicated for 72 h. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. indicated control (n=5-12).
Human Alpha Synuclein Overexpressioin Plasmid, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ddddk+tagged+fusion+protein+overexpression+e+coli+lysate/Human+SNCA%2Falpha-Synuclein+Gene+ORF+cDNA+clone+expression+plasmid%2C+N-GFPSpark+tag/bio_rxiv__2021__02__17__431525-196-5-11
Average 91 stars, based on 1 article reviews
human alpha synuclein overexpressioin plasmid - by Bioz Stars, 2026-10
91/100 stars
  Buy from Supplier
gcet2 overexpression lysate native  (Novus Biologicals)
N/A
   Buy from Supplier
c9orf23 overexpression lysate native  (Novus Biologicals)
N/A
   Buy from Supplier
kcnip4 overexpression lysate native  (Novus Biologicals)
N/A
   Buy from Supplier
ccny overexpression lysate native  (Novus Biologicals)
N/A
   Buy from Supplier
p38 overexpression lysate native  (Novus Biologicals)
N/A
   Buy from Supplier
il 17d overexpression lysate native  (Novus Biologicals)
N/A
   Buy from Supplier
slc26a7 overexpression lysate native  (Novus Biologicals)
N/A
   Buy from Supplier
kv6 4 overexpression lysate native  (Novus Biologicals)
N/A
   Buy from Supplier
integrin beta 1 cd29 overexpression lysate native  (Novus Biologicals)
N/A
   Buy from Supplier
rad17 overexpression lysate native  (Novus Biologicals)
N/A
   Buy from Supplier

Image Search Results


(A) The BZ555 strain was pretreated with 6-OHDA (30 mM) for 1 h then transferred to the plates with 5-Fluorouridine (0.04 mg/mL) and indicated concentrations of Rg3 or L-DOPA (10 µM) for 72 h. The fluorescence intensity of dopaminergic neurons was measured. (B) The eggs of NL5901 strain were treated with indicated concentrations of Rg3 or L-DOPA (10 µM). After growing to the L4 stage, the nematodes were treated with 5-Fluorouridine (0.04 mg/mL) for 12 days. The fluorescence intensity of α-synuclein protein in nematodes was measured. (C) The N2 strain was pretreated 6-OHDA (30 mM) for 1 h then transferred to the plates with Rg3 (90 µM) for 36h. The cardiolipin content was measured by NAO (10 μM) fluorescence signaling. (D-E) The BZ555 strain was cultured for three generations on culture plates with bacteria containing the indicated RNAi in the presence of Rg3 (90 µM). (D) Then the fluorescence intensity of dopaminergic neurons was measured. (E) The BZ555 strain was pretreated with 6-OHDA (30 mM) for 1 h then transferred to the plates with Rg3 (90 µM), 5-Fluorouridine (0.04 mg/mL) and cardiolipin (100 µg/ml) as indicated for 72 h. (F) The SJ4100 strain was treated with 6-OHDA (30 mM), 5-Fluorouridine (0.04 mg/mL) and Rg3 (90 µM) as indicated for different durations. The fluorescence representing UPR mt was measured. (G) The SJ4100 strain was treated with Rg3 (90 µM) and 6-OHDA (30 mM) as indicated for 12 h, the fluorescence representing UPR mt was measured. (H) The BZ555 strain fed with hsp-6 RNAi was pretreated with 6-OHDA (30 mM) for 1 h then transferred to the plates with 5-Fluorouridine (0.04 mg/mL) and Rg3 (90 µM) as indicated for 72 h. (I) The BZ555 strain fed with hsp-6 RNAi was pretreated with 6-OHDA (30 mM) for 1 h then transferred to the plates with 5-Fluorouridine (0.04 mg/mL), Rg3 (90 µM) and cardiolipin (100 µg/ml) as indicated for 72 h. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. indicated control (n=5-12).

Journal: bioRxiv

Article Title: Discovery of a molecular glue that enhances UPR mt to restore proteostasis via TRKA-GRB2-EVI1-CRLS1 axis

doi: 10.1101/2021.02.17.431525

Figure Lengend Snippet: (A) The BZ555 strain was pretreated with 6-OHDA (30 mM) for 1 h then transferred to the plates with 5-Fluorouridine (0.04 mg/mL) and indicated concentrations of Rg3 or L-DOPA (10 µM) for 72 h. The fluorescence intensity of dopaminergic neurons was measured. (B) The eggs of NL5901 strain were treated with indicated concentrations of Rg3 or L-DOPA (10 µM). After growing to the L4 stage, the nematodes were treated with 5-Fluorouridine (0.04 mg/mL) for 12 days. The fluorescence intensity of α-synuclein protein in nematodes was measured. (C) The N2 strain was pretreated 6-OHDA (30 mM) for 1 h then transferred to the plates with Rg3 (90 µM) for 36h. The cardiolipin content was measured by NAO (10 μM) fluorescence signaling. (D-E) The BZ555 strain was cultured for three generations on culture plates with bacteria containing the indicated RNAi in the presence of Rg3 (90 µM). (D) Then the fluorescence intensity of dopaminergic neurons was measured. (E) The BZ555 strain was pretreated with 6-OHDA (30 mM) for 1 h then transferred to the plates with Rg3 (90 µM), 5-Fluorouridine (0.04 mg/mL) and cardiolipin (100 µg/ml) as indicated for 72 h. (F) The SJ4100 strain was treated with 6-OHDA (30 mM), 5-Fluorouridine (0.04 mg/mL) and Rg3 (90 µM) as indicated for different durations. The fluorescence representing UPR mt was measured. (G) The SJ4100 strain was treated with Rg3 (90 µM) and 6-OHDA (30 mM) as indicated for 12 h, the fluorescence representing UPR mt was measured. (H) The BZ555 strain fed with hsp-6 RNAi was pretreated with 6-OHDA (30 mM) for 1 h then transferred to the plates with 5-Fluorouridine (0.04 mg/mL) and Rg3 (90 µM) as indicated for 72 h. (I) The BZ555 strain fed with hsp-6 RNAi was pretreated with 6-OHDA (30 mM) for 1 h then transferred to the plates with 5-Fluorouridine (0.04 mg/mL), Rg3 (90 µM) and cardiolipin (100 µg/ml) as indicated for 72 h. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. indicated control (n=5-12).

Article Snippet: HEK293 cells were transfected with human alpha-synuclein overexpressioin plasmid (Cat: HG12093-ANG, Sinobiological, Beijing, China) using Lipo3000.

Techniques: Fluorescence, Cell Culture

(A) The BZ555 strain was pretreated with 6-OHDA (30 mM) for 1 h then transferred to the plates with 5-Fluorouridine (0.04 mg/mL) and indicated concentrations of Rg3 or L-DOPA (10 µM) for 72 h. The fluorescence intensity of dopaminergic neurons was measured. Scale bar: 40 µm. (B) The eggs of NL5901 strain were treated with indicated concentrations of Rg3 or L-DOPA (10 µM). After growing to the L4 stage, the nematodes were treated with 5-Fluorouridine (0.04 mg/mL) for 12 days. The fluorescence intensity of α-synuclein protein in nematodes was measured. Scale bar: 40 µm. (C) The N2 strain was pretreated 6-OHDA (30 mM) for 1 h then transferred to the plates with Rg3 (90 µM) for 36h. The cardiolipin content was measured by NAO (10 μM) fluorescence signaling. Scale bar: 100 µm. (D-E) The eggs of NL5901 strain were treated with indicated concentrations of Rg3 or L-DOPA (10 µM). After growing to the L4 stage, the nematodes were treated with 5-Fluorouridine (0.04 mg/mL). After 60 h, the number of nematodes was recorded every day. ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. indicated control (A-C: n=5-12; D-E: n=29-38).

Journal: bioRxiv

Article Title: Discovery of a molecular glue that enhances UPR mt to restore proteostasis via TRKA-GRB2-EVI1-CRLS1 axis

doi: 10.1101/2021.02.17.431525

Figure Lengend Snippet: (A) The BZ555 strain was pretreated with 6-OHDA (30 mM) for 1 h then transferred to the plates with 5-Fluorouridine (0.04 mg/mL) and indicated concentrations of Rg3 or L-DOPA (10 µM) for 72 h. The fluorescence intensity of dopaminergic neurons was measured. Scale bar: 40 µm. (B) The eggs of NL5901 strain were treated with indicated concentrations of Rg3 or L-DOPA (10 µM). After growing to the L4 stage, the nematodes were treated with 5-Fluorouridine (0.04 mg/mL) for 12 days. The fluorescence intensity of α-synuclein protein in nematodes was measured. Scale bar: 40 µm. (C) The N2 strain was pretreated 6-OHDA (30 mM) for 1 h then transferred to the plates with Rg3 (90 µM) for 36h. The cardiolipin content was measured by NAO (10 μM) fluorescence signaling. Scale bar: 100 µm. (D-E) The eggs of NL5901 strain were treated with indicated concentrations of Rg3 or L-DOPA (10 µM). After growing to the L4 stage, the nematodes were treated with 5-Fluorouridine (0.04 mg/mL). After 60 h, the number of nematodes was recorded every day. ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. indicated control (A-C: n=5-12; D-E: n=29-38).

Article Snippet: HEK293 cells were transfected with human alpha-synuclein overexpressioin plasmid (Cat: HG12093-ANG, Sinobiological, Beijing, China) using Lipo3000.

Techniques: Fluorescence

(A) HEK293 cells were transfected with GFP-tagged α-synuclein plasmid, and then treated with Rg3 (5 μM) or L-DOPA (10 μM) for 48 h. The amount of α-synuclein protein level was measured by green fluorescence signals. Scale bar: 100 µm. (B-C) SH-SY5Y cells were treated with Rg3 (5 μM) and 6-OHDA (60 μM) for 24 h: (B) Fluorescence intensity of cells was measured by NAO (5 μM) fluorescence signaling. Scale bar: 100 µm. (C) Total proteins were extracted and subjected to western blot with indicated antibodies. All experiments were repeated at least three times, * p < 0.05, ** p < 0.01 vs. indicated control.

Journal: bioRxiv

Article Title: Discovery of a molecular glue that enhances UPR mt to restore proteostasis via TRKA-GRB2-EVI1-CRLS1 axis

doi: 10.1101/2021.02.17.431525

Figure Lengend Snippet: (A) HEK293 cells were transfected with GFP-tagged α-synuclein plasmid, and then treated with Rg3 (5 μM) or L-DOPA (10 μM) for 48 h. The amount of α-synuclein protein level was measured by green fluorescence signals. Scale bar: 100 µm. (B-C) SH-SY5Y cells were treated with Rg3 (5 μM) and 6-OHDA (60 μM) for 24 h: (B) Fluorescence intensity of cells was measured by NAO (5 μM) fluorescence signaling. Scale bar: 100 µm. (C) Total proteins were extracted and subjected to western blot with indicated antibodies. All experiments were repeated at least three times, * p < 0.05, ** p < 0.01 vs. indicated control.

Article Snippet: HEK293 cells were transfected with human alpha-synuclein overexpressioin plasmid (Cat: HG12093-ANG, Sinobiological, Beijing, China) using Lipo3000.

Techniques: Transfection, Plasmid Preparation, Fluorescence, Western Blot